Search results for "ISM [Radio continuum]"
showing 10 items of 703 documents
ERa dimerization: a key factor for the weak estrogenic activity of an ERa modulator unable to compete with estradiol in binding assays
2016
PMID: 27400858; International audience; AbstractEstrothiazine (ESTZ) is a weak estrogen sharing structural similarities with coumestrol. ESTZ failed to compete with [3H]17β-estradiol ([3H]17β-E2) for binding to the estrogen receptor α (ERα), questioning its ability to interact with the receptor. However, detection by atomic force spectroscopy (AFS) of an ESTZ-induced ERα dimerization has eliminated any remaining doubts. The effect of the compound on the proliferation of ERα-positive and negative breast cancer cells confirmed the requirement of the receptor. The efficiency of ESTZ in MCF-7 cells was weak without any potency to modify the proliferation profile of estradiol and coumestrol. Gro…
Genomic and non-genomic mechanisms of action of thyroid hormones and their catabolite 3,5-diiodo-l-thyronine in Mammals
2020
Since the realization that the cellular homologs of a gene found in the retrovirus that contributes to erythroblastosis in birds (v-erbA), i.e. the proto-oncogene c-erbA encodes the nuclear receptors for thyroid hormones (THs), most of the interest for THs focalized on their ability to control gene transcription. It was found, indeed, that, by regulating gene expression in many tissues, these hormones could mediate critical events both in development and in adult organisms. Among their effects, much attention was given to their ability to increase energy expenditure, and they were early proposed as anti-obesity drugs. However, their clinical use has been strongly challenged by the concomita…
Penalized classification for optimal statistical selection of markers from high-throughput genotyping: application in sheep breeds
2018
The identification of individuals’ breed of origin has several practical applications in livestock and is useful in different biological contexts such as conservation genetics, breeding and authentication of animal products. In this paper, penalized multinomial regression was applied to identify the minimum number of single nucleotide polymorphisms (SNPs) from high-throughput genotyping data for individual assignment to dairy sheep breeds reared in Sicily. The combined use of penalized multinomial regression and stability selection reduced the number of SNPs required to 48. A final validation step on an independent population was carried out obtaining 100% correctly classified individuals. …
Sustained activation of sphingomyelin synthase by 2-hydroxyoleic acid induces sphingolipidosis in tumor cells
2013
The mechanism of action of 2-hydroxyoleic acid (2OHOA), a potent antitumor drug, involves the rapid and specific activation of sphingomyelin synthase (SMS), leading to a 4-fold increase in SM mass in tumor cells. In the present study, we investigated the source of the ceramides required to sustain this dramatic increase in SM. Through radioactive and fluorescent labeling, we demonstrated that sphingolipid metabolism was altered by a 24 h exposure to 2OHOA, and we observed a consistent increase in the number of lysosomes and the presence of unidentified storage materials in treated cells. Mass spectroscopy revealed that different sphingolipid classes accumulated in human glioma U118 cells af…
Mitochondrial CB1 receptors regulate neuronal energy metabolism
2012
The mammalian brain is one of the organs with the highest energy demands, and mitochondria are key determinants of its functions. Here we show that the type-1 cannabinoid receptor (CB(1)) is present at the membranes of mouse neuronal mitochondria (mtCB(1)), where it directly controls cellular respiration and energy production. Through activation of mtCB(1) receptors, exogenous cannabinoids and in situ endocannabinoids decreased cyclic AMP concentration, protein kinase A activity, complex I enzymatic activity and respiration in neuronal mitochondria. In addition, intracellular CB(1) receptors and mitochondrial mechanisms contributed to endocannabinoid-dependent depolarization-induced suppres…
Tourism marketing and local celebrations: A case study in Valencia’s Fallas
2020
El artículo pretende mostrar la necesidad de emplear estrategias de marketing turístico en la oferta de la Fiesta de las Fallas que tienen lugar en Valencia (España), declaradas Patrimonio Cultural Inmaterial de la Humanidad en 2016. Se desarrolla la teoría acerca de esta herramienta, argumentando las características y capacidades del evento y fundamentando su importancia como pieza clave en el desarrollo del turismo cultural valenciano. Los datos recogidos y las entrevistas abiertas realizadas manifiestan que la Fiesta de las Fallas debe considerarse un producto turístico y emplear estrategias que muestren la autenticidad y singularidad de Valencia como destino turístico. The article aims …
Raising awareness of multilingualism as lived – in the context of teaching English as a foreign language
2020
Tässä artikkelissa esitellään mahdollisuuksia herätellä kielen oppijoiden kieli- ja kulttuuritietoisuutta. Esitellyt mahdollisuudet pohjautuvat 24 empiirisen tutkimuksen kriittiseen arviointiin. Tutkimuksissa käytettiin kuvataiteisiin pohjautuvia visuaalisia menetelmiä. Tällaisten menetelmien avulla on mahdollista pohtia aikaisempia kokemuksia tai kuvitella tulevaisuutta ja näin reflektoida erilaisia monikielisyyden kokemuksia. Näitä voivat olla identiteetin rakentumiseen liittyvät kysymykset, käsitykset eri kielten käytöstä tai tulevaisuuden unelmat. Arviointimme perusteella esitämme, että niitä tehtäviä, joita käytettiin tutkimuksissa, voidaan soveltaa eri tavoin erilaisille vieraan kiele…
The Catholic Nation under Liberalism. Perspectives on Religious Unity in Liberal Spain, 1808-1868
2015
El artículo estudia los significados históricos que tuvo la afirmación de la unidad religiosa de la nación española en el liberalismo decimonónico. A partir de las premisas historiográficas que han renovado las reflexiones en torno a las relaciones entre religión, política y nación, los autores analizan la herencia plural de los ilustrados del último tercio del siglo XVIII y el sentido de la homogeneidad confesional de la nación soberana, esbozada por la Constitución de 1812. La confesionalidad religiosa no impidió las posibilidades de emancipación individual. Por el contrario, el primer liberalismo tendió a un reforzamiento mutuo de las identidades religiosa y nacional, al tiempo que confi…
Challenges and Opportunities on Lipid Metabolism Disorders Diagnosis and Therapy: Novel Insights and Future Perspective
2021
Dyslipidemia has been globally recognized, for almost seven decades, as one of the most important risk factors for the development and complications of atherosclerotic cardiovascular disease (ASCVD) [...]